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Image Search Results
Journal: International journal of oncology
Article Title: Mast cell chymase promotes angiogenesis and lymphangiogenesis mediated by activation of melanoma inhibitory activity gene family members in oral squamous cell carcinoma.
doi: 10.3892/ijo.2020.4996
Figure Lengend Snippet: Figure 5. Effects of chymase on angiogenesis and lymphangiogenesis in OSCC cells. Effects of chymase and MIA gene family member siRNA transfection on (A) VEGF‑A, (B) VEGF‑C, (C) VEGF‑C and (D) PDGFB secretion in OSCC cells. Changes in the (E and F) adhesive and (G and H) transmigra tory abilities of HSC3 cells to endothelial cells following chymase treatment and/or MIA gene family member siRNA transfection. *P<0.05. OSCC, oral squamous cell carcinoma; MIA, melanoma inhibitory activity; TANGO, transport and Golgi organization protein 1; PDGFB, platelet‑derived growth factor β polypeptide; siRNA, small interfering RNA; VEGF, vascular endo thelial growth factor.
Article Snippet: ELISA kits were used to analyze MIA (cat. no. 11976826001; Roche Diagnostics), MIA2 (cat. no. LS‐F16959; LifeSpan BioSciences, Inc.), TANGO/MIA3 (cat. no. LS‐F52248; LifeSpan BioSciences, Inc.), VEGF‐A (cat. no. RAB0508; Calbiochem; Merck KGaA), VEGF‐C (cat. no. DVEC00; R&D Systems, Inc.), VEGF‐D (cat. no. DVED00; R&D Systems, Inc.) and
Techniques: Transfection, Adhesive, Activity Assay, Small Interfering RNA
Journal: Bone
Article Title: Preservation of Type H Vessels and Osteoblasts by Enhanced Preosteoclast Platelet-Derived Growth Factor type BB Attenuates Glucocorticoid-Induced Osteoporosis in Growing Mice
doi: 10.1016/j.bone.2018.05.025
Figure Lengend Snippet: (A-B) Representative images of immunofluorescence staining of platelet-derived growth factor type BB (PDGF-BB) (green) and tartrate-resistant acid phosphatase (TRAP) (red) with quantification of number of PDGF-BB+ TRAP+ cells (yellow) in femoral primary (A) and secondary spongiosa (B) of 6 week old Ctsk+/+ wild type (WT) and Ctsk−/− mice injected with either vehicle (veh) or prednisolone 10 mg/m2/day (pred) for 4 weeks. DAPI stains nuclei blue. Scale bar, 50 μm. (C) Bone marrow PDGF-BB concentration as analyzed by ELISA. n = 6 per group. Data shown as mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001.
Article Snippet: We performed PDGF-BB ELISA analysis of bone marrow supernatant using a
Techniques: Immunofluorescence, Staining, Derivative Assay, Injection, Concentration Assay, Enzyme-linked Immunosorbent Assay